Nanopartz™ Gold Nanoparticles for Lateral Flow

 

Functionalized gold nanoparticle with Nanopartz™ covalently bonded polymer (top) and Nanopartz™ covalent MUTAB (bottom). Thickness of polymer is less than the size variablity of the gold nanoparticle.   Conjugations (Ordering Abbreviation) Amine TA Biotin TB Carboxyl TC Methyl TM Neutravidin TN NHS TNHS Streptavidin TS Secondary antibodies

 

Composition

These in vitro gold nanoparticles for in vitro dark field, bright field, and fluorescence imaging come in numerous conjugations:

Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.

1. Amine
2. Biotin
3. Carboxyl
4. Methyl
5. Neutravidin
6. Streptavidin
7. Azide

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

1. Goat Anti-Human IgG
2. Goat Anti-Mouse IgG
3. Goat Anti-Rabitt IgG
4. Goat Anti-Human IgA
5. Goat Anti-Human IgM
6. Protein A

Custom Conjugations are also available including HRP and GSH.

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

 

These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field, bright field, and fluorescence microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products.

Nanopartz™ Gold Nanoparticles for Lateral Flow Citrate spherical gold nanoparticles with adsorbed conjugates Stability (salt,pH,chemicals) High Low Scattering linewidth Narrow (monodisperse) Broad (polydisperse) Multilabeling Yes (500nm-2100nm) Limited (520nm-600nm) Nonspecific binding Very low High pH and salt resistance High Medium Sterilization Radiation Sodium Azide Site specific antibody conjugation Yes No

• Utilizing our exclusive license to Dr. Cathy Murphy's Nanorod Technology, our nanorods are highly accurate and monodisperse in size, shape, and batch to batch uniformity.
• Extremely stable. Shelf life is greater than six months.
• Covalent bonds.
• High anisotropy.
• Low cytotoxicity. Gold is FDA approved for rheumatoid arthritis.
• Nanorod to sphere shape changing on high energy pulsed lasers.
• Bright Fluorescence - intense emission provides superior sensitivity and requires less conjugate.
• Narrow Emission spectra - covalent bonds ensure no bleed through.
• Excellent photostability - exceptional resistance to photobleaching enables fluorescence imaging uner the most demanding conditions.
• Buffer Stability - stable from pH 4-9.
• Instrument cability - excitation and emission spectra correspond with filter sets and laser setting of all popular fluorescence instrumentation.

• Customer can focus on research and not on Nanopartz™ expertise
• Quick turnaround

How much thickness does your proprietary capping agent add?

It is less than the variablility of the size of the nanoparticles themselves, so it is unmeasurable.

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solvent you add.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

Can I suspend in silicon oil?

No

Will the SPR wavelength shift with different solvents?

The SPR is a function of the index of refraction of the host solvent in which the nanoparticles are suspended. Nanorods are the most sensitive and are therefore a great tool for measuring the index of refraction of the host.

What is the shelf life if I never open the package?

1 year.

Should I store in the refrigerator?

Yes.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University