Gold Nanoparticle-Based Darkfield Imaging: Advanced in vitro Solutions

Darkfield imaging with gold nanoparticles revolutionizes in vitro research and diagnostics. Nanopartz delivers high-quality gold nanoparticles optimized for precise imaging applications, enabling breakthroughs in biomedical sciences.

 

A visual representation of a Dark Field Microscope Setup

A visual representation of a Dark Field Microscope Setup

  

Introduction to Darkfield Imaging with Gold Nanoparticles

Darkfield imaging is a powerful technique that enhances the visibility of nanoscale particles in complex biological environments. By using gold nanoparticles, researchers achieve superior contrast and resolution, enabling detailed observation of cellular processes and molecular interactions.

Why Choose Gold Nanoparticles for Darkfield Imaging?

  • High scattering efficiency for superior visibility.
  • Biocompatible and functionalizable for various biological applications.
  • Stability and reproducibility across imaging experiments.

Applications of Gold Nanoparticle-Based Darkfield Imaging

  • Biological Imaging
    • Real-time tracking of cellular processes.
    • Enhanced imaging of cellular structures, such as membranes and nuclei.
    • Visualization of molecular interactions using functionalized nanoparticles.
  • Material Science
    • Investigation of material compositions and interactions.
    • Analysis of surface plasmon resonance (SPR) effects.
  • Disease Diagnostics
    • Early detection of cancer markers.
    • Study of pathogen-host interactions.

Nanopartz: Your Partner in Advanced Darkfield Imaging Nanopartz offers premium gold nanoparticles tailored for darkfield imaging applications. Our products are characterized by:

  • Unmatched Sensitivity: The high scattering properties of gold nanoparticles allow detection of structures as small as a few nanometers.
  • Dynamic Post-Processing Options: Advanced imaging techniques reveal color dynamics that bring a new dimension to microscopy.
  • Robust Quality Assurance: Each batch is rigorously tested to ensure particle uniformity and functional integrity.
  • Custom Solutions: Tailored nanoparticle solutions to meet the unique needs of your research.
 

Optical Hyperspectral Microscopy of Specific Nanoparticle Uptake and Localization

Optical Hyperspectral Microscopy of Specific Nanoparticle Uptake and Localization

  

 

 

Dark Field Image of Nanopartz Gold Nanowires

Dark Field Image of Nanopartz Gold Nanowires

  

 

 

Dark Field Image of Nanopartz Spherical Gold Nanoparticles

Dark Field Image of Nanopartz Spherical Gold Nanoparticles

  

 

Conclusion

Nanopartz fluorophore-labeled gold nanoparticles are a game-changer in fluorescence microscopy, especially when paired with Miniflux Microscopy. Their ability to push the resolution limits and provide stable, bright fluorescence signals is key to advancing biological research. Whether for cellular imaging or tracking molecular dynamics, these nanoparticles offer unparalleled performance, making them indispensable in modern scientific endeavors.

References

A few of the many publications using Nanopartz products in Dark Field Microscopy.

  1. Calvo, R., A. Thon, A. Saad, A. Salvador-Matar, et al. "Size Characterization of Plasmonic Nanoparticles with Dark-Field Single Particle Spectrophotometry." Scientific Reports, vol. 12, no. 1, 2022, pp. 1-10. Nature.

  2. Xiao, L., Y.X. Qiao, Y. He, and E.S. Yeung. "Three-Dimensional Orientational Imaging of Nanoparticles with Darkfield Microscopy." Analytical Chemistry, vol. 82, no. 14, 2010, pp. 5268-5274. ACS Publications.

  3. Abadie, C., M. Liu, Y. Prado, and O. Pluchery. "Hyperspectral Dark-Field Optical Microscopy Correlated to Atomic Force Microscopy for the Analysis of Single Plasmonic Nanoparticles: Tutorial." Journal of the Optical Society of America A, vol. 41, no. 1, 2024, pp. 87-103. OPG Optica. [PDF available at optica.org]

  4. Liu, X., Z. Wu, Q. Zhang, W. Zhao, and C. Zong. "Single Gold Nanoparticle-Based Colorimetric Detection of Picomolar Mercury Ion with Dark-Field Microscopy." Analytical Chemistry, vol. 88, no. 22, 2016, pp. 11042-11048. ACS Publications.

  5. Calvo, R., A. Thon, A. Saad, A. Salvador-Matar, et al. "Size Characterization of Plasmonic Nanoparticles with Dark-Field Single Particle Spectrophotometry." Scientific Reports, vol. 12, no. 1, 2022, pp. 1-10. Nature.

  6. Artur, C.G., T. Womack, F. Zhao, J.L. Eriksen, et al. "Plasmonic Nanoparticle-Based Expansion Microscopy with Surface-Enhanced Raman and Dark-Field Spectroscopic Imaging." Biomedical Optics Express, vol. 9, no. 4, 2018, pp. 1800-1814. OPG Optica. [PDF available at optica.org]

 

Go here to purchase Functionalized Gold Nanoparticles

Go here to purchase Gold Nanoparticles for Cell Uptake

Go here to purchase Gold Nanoparticles for Nuclei Uptake

 

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