Nanopartz Functionalized Gold Nanoparticles for NiNTA and HisTag Chemistries

Nanopartz Gold Nanoparticles, conjugated specifically for NiNTA and HisTag applications, utilize the complete line of Accurate Spherical Gold Nanoparticles and Gold Nanorods conjugated via a proprietary covalent polymer bridge, or PEG, to the most popular click chemistry active groups including NiNTA and HisTag. This family of products is well known for its resistant to temperature, salt, and pH, and for its exceptional loading capability. These conjugated particles can be used in a multitude of applications, including diagnostics, imaging, particularly in adverse environments. These particles are guaranteed not to aggregate. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. Typical volume is 1mL at a concentration of 50 OD. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. In all cases, the loading of the target is determined.

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Product Specifications

Part #CN11 Nanopartz NiNTA and HisTag Chemistry Spherical Gold Nanoparticles

Part # CN11-	Diameter (nm)	Peak SPR Wavelength (nm)	NPS/ml	Molarity (pM)	Moles	Molar Ext. (M-1cm-1)	Absorption Molar Ext. (M-1cm-1)	Scattering Molar Ext. (M-1cm-1)	Size Dispersity %PDI	Size Accuracy (+/- nm)
1.8	1.8	n/a	4.25E+16	7.10E+07	7.09E-08	7.05E+05	7.05E+05	0.00E+00	<35%	0.1
2.2	2.2	n/a	2.33E+16	3.90E+07	3.88E-08	1.29E+06	1.29E+06	0.00E+00	<25%	0.1
3	3	n/a	9.19E+15	1.50E+07	1.53E-08	3.26E+06	3.26E+06	0.00E+00	<20%	0.1
4	4	n/a	3.88E+15	6.50E+06	6.46E-09	7.74E+06	7.74E+06	0.00E+00	<20%	1
5	5	512	1.99E+15	3.30E+06	3.31E-09	1.51E+07	1.51E+07	0.00E+00	<20%	2
10	10	516	2.48E+14	4.10E+05	4.14E-10	1.21E+08	1.21E+08	0.00E+00	<15%	2
15	15	518	7.35E+13	1.20E+05	1.23E-10	4.08E+08	4.08E+08	0.00E+00	<15%	2
20	20	520	3.10E+13	5.20E+04	5.17E-11	9.67E+08	9.67E+08	0.00E+00	<10%	2
25	25	521	1.59E+13	2.60E+04	2.65E-11	1.89E+09	1.89E+09	0.00E+00	<10%	2
30	30	523	9.19E+12	1.50E+04	1.53E-11	3.26E+09	3.26E+09	0.00E+00	<6%	2
35	35	526	5.79E+12	9.60E+03	9.65E-12	5.18E+09	5.18E+09	0.00E+00	<6%	2
40	40	527	3.88E+12	6.50E+03	6.46E-12	7.74E+09	7.72E+09	2.24E+07	<4%	2
45	45	529	2.72E+12	4.50E+03	4.54E-12	1.10E+10	1.08E+10	2.03E+08	<4%	2
50	50	531	1.99E+12	3.30E+03	3.31E-12	1.51E+10	1.45E+10	5.88E+08	<4%	2
55	55	533	1.49E+12	2.50E+03	2.49E-12	2.01E+10	1.88E+10	1.30E+09	<4%	2
60	60	536	1.15E+12	1.90E+03	1.91E-12	2.61E+10	2.36E+10	2.48E+09	<4%	2
65	65	539	9.04E+11	1.50E+03	1.51E-12	3.32E+10	2.89E+10	4.33E+09	<4%	2
70	70	542	7.23E+11	1.20E+03	1.21E-12	4.15E+10	3.44E+10	7.09E+09	<4%	2
75	75	545	5.88E+11	9.80E+02	9.80E-13	5.10E+10	4.00E+10	1.10E+10	<4%	2
80	80	549	4.85E+11	8.10E+02	8.08E-13	6.19E+10	4.54E+10	1.65E+10	<4%	2
85	85	553	4.04E+11	6.70E+02	6.73E-13	7.42E+10	5.03E+10	2.39E+10	<4%	2
90	90	558	3.40E+11	5.70E+02	5.67E-13	8.81E+10	5.44E+10	3.37E+10	<4%	2
95	95	563	2.89E+11	4.80E+02	4.82E-13	1.04E+11	5.72E+10	4.65E+10	<4%	2
100	100	569	2.48E+11	4.10E+02	4.14E-13	1.21E+11	5.82E+10	6.27E+10	<4%	2
150	150	612	7.35E+10	1.20E+02	1.23E-13	4.08E+11	0.00E+00	4.08E+11	<4%	10
200	200	n/a	3.10E+10	5.20E+01	5.17E-14	9.67E+11	0.00E+00	9.67E+11	<4%	10
500	500	n/a	1.99E+09	3.30E+00	3.31E-15	1.51E+13	0.00E+00	1.51E+13	<4%	50
1000	1000	n/a	2.48E+08	4.10E-01	4.14E-16	1.21E+14	0.00E+00	1.21E+14	<4%	100
1500	1500	n/a	7.35E+07	1.20E-01	1.23E-16	4.08E+14	0.00E+00	4.08E+14	<4%	100

Optical Density >= 50, 5 for sizes >=500nm

Wt conc = 2.5mg/mL, 1mL volume typical

Wt % = 0.25%

ppm = 2500

%PDI = Std Dev/Size

See Tech Note TN801 for definition of terms and method of analysis

All specs typical. May vary batch to batch. Exact values are measured for each batch

Shape monodispersity (% spheres) > 99.9%

Solution default is 18MEG DI water

Residual Chemicals < 0.1%

Part #CN12 Nanopartz NiNTA and HisTag Chemistry Gold Nanorods

Part # CN12-	Diameter (nm)	Length (nm)	Peak SPR Wave (nm)	Aspect Ratio	OD SPR (AU)	Peak LSPR Wave (nm)	OD LSPR (AU)	Nanorods /mL	Wt. conc (ug/ml)	Wt. %	PPM	Molarity (nM)	Picomoles	SPR Molar Ext. (M-1cm-1)	LSPR Molar Ext. (M-1cm-1)	Peak SPR (nm)	SPR Linewidth 80% (nm)
5-808	5	21	808	4.2	50	510	10	2.39E+14	1750	0.18%	1750	398.72	3.99E+02	1.25E+08	2.51E+07	794-829	100
5-780	5	19	780	3.8	50	510	10	2.67E+14	1750	0.18%	1750	444.72	4.45E+02	1.12E+08	2.25E+07	765-794	100
5-700	5	15	700	3	50	510	10	3.47E+14	1750	0.18%	1750	578.14	5.78E+02	8.65E+07	1.73E+07	675-725	100

10-2100	10	175	2100	17.5	50	510	10	6.73E+12	1750	0.18%	1750	11.21	1.12E+01	4.46E+09	8.92E+08	1900-2300	300
10-1400	10	102	1400	10.2	50	510	10	1.17E+13	1750	0.18%	1750	19.47	1.95E+01	2.57E+09	5.14E+08	1300-1500	150
10-1200	10	81	1200	8.1	50	510	10	1.48E+13	1750	0.18%	1750	24.67	2.47E+01	2.03E+09	4.05E+08	1132-1300	150
10-1064	10	67	1064	6.7	50	510	10	1.81E+13	1750	0.18%	1750	30.13	3.01E+01	1.66E+09	3.32E+08	1022-1132	150
10-980	10	59	980	5.9	50	510	10	2.09E+13	1750	0.18%	1750	34.91	3.49E+01	1.43E+09	2.86E+08	965-1022	150
10-950	10	55	950	5.5	50	510	10	2.22E+13	1750	0.18%	1750	37	3.70E+01	1.35E+09	2.70E+08	925-965	150
10-900	10	50	900	5	50	510	10	2.47E+13	1750	0.18%	1750	41.11	4.11E+01	1.22E+09	2.43E+08	875-925	150
10-850	10	45	850	4.5	50	510	10	2.78E+13	1750	0.18%	1750	46.25	4.63E+01	1.08E+09	2.16E+08	829-875	100
10-808	10	41	808	4.1	50	510	10	3.10E+13	1750	0.18%	1750	51.68	5.17E+01	9.68E+08	1.94E+08	794-829	75
10-780	10	38	780	3.8	50	510	10	3.36E+13	1750	0.18%	1750	56.06	5.61E+01	8.92E+08	1.78E+08	765-794	65
10-750	10	35	750	3.5	50	510	10	3.70E+13	1750	0.18%	1750	61.67	6.17E+01	8.11E+08	1.62E+08	725-765	50
10-700	10	29	700	2.9	50	510	10	4.44E+13	1750	0.18%	1750	74	7.40E+01	6.76E+08	1.35E+08	675-725	40
10-650	10	24	650	2.4	50	510	10	5.55E+13	1750	0.18%	1750	92.5	9.25E+01	5.41E+08	1.08E+08	625-675	40
10-600	10	19	600	1.9	50	510	10	7.40E+13	1750	0.18%	1750	123.34	1.23E+02	4.05E+08	8.11E+07	575-625	40

25-1400	25	245	1400	9.8	50	514	15	1.12E+12	2500	0.25%	2500	1.86	1.86E+00	2.69E+10	8.06E+09	1232-1500	150
25-1064	25	137	1064	5.5	50	514	15	2.05E+12	2500	0.25%	2500	3.42	3.42E+00	1.46E+10	4.38E+09	1022-1232	150
25-980	25	119	980	4.8	50	514	15	2.39E+12	2500	0.25%	2500	3.98	3.98E+00	1.26E+10	3.77E+09	965-1022	150
25-950	25	102	950	4.1	50	514	15	2.82E+12	2500	0.25%	2500	4.7	4.70E+00	1.06E+10	3.19E+09	925-965	150
25-900	25	96	900	3.8	50	514	15	3.01E+12	2500	0.25%	2500	5.02	5.02E+00	9.95E+09	2.99E+09	875-925	150
25-850	25	93	850	3.7	50	514	15	3.12E+12	2500	0.25%	2500	5.2	5.20E+00	9.61E+09	2.88E+09	829-875	150
25-808	25	90	808	3.6	50	514	15	3.24E+12	2500	0.25%	2500	5.39	5.39E+00	9.27E+09	2.78E+09	794-829	150
25-780	25	87	780	3.5	50	514	15	3.36E+12	2500	0.25%	2500	5.6	5.60E+00	8.93E+09	2.68E+09	765-794	150
25-750	25	85	750	3.4	50	514	15	3.45E+12	2500	0.25%	2500	5.75	5.75E+00	8.70E+09	2.61E+09	725-765	65
25-700	25	75	700	3	50	514	15	3.96E+12	2500	0.25%	2500	6.61	6.61E+00	7.57E+09	2.27E+09	675-725	60
25-650	25	71	650	2.8	50	514	15	4.22E+12	2500	0.25%	2500	7.03	7.03E+00	7.11E+09	2.13E+09	625-675	60
25-600	25	57	600	2.3	50	514	15	5.43E+12	2500	0.25%	2500	9.05	9.05E+00	5.52E+09	1.66E+09	575-625	60
25-550	25	34	550	1.4	50	514	15	1.01E+13	2500	0.25%	2500	16.91	1.69E+01	2.96E+09	8.87E+08	525-575	60

40-1064	40	208	850	5.2	50	520	20	5.30E+11	2500	0.25%	2500	0.88	8.84E-01	5.66E+10	2.26E+10	1022-1232	150
40-980	40	180	850	4.5	50	520	20	6.19E+11	2500	0.25%	2500	1.03	1.03E+00	4.84E+10	1.94E+10	875-1022	150
40-850	40	148	850	3.7	50	520	20	7.67E+11	2500	0.25%	2500	1.28	1.28E+00	3.91E+10	1.57E+10	829-875	150
40-808	40	134	808	3.4	50	520	20	8.56E+11	2500	0.25%	2500	1.43	1.43E+00	3.51E+10	1.40E+10	794-829	150
40-780	40	124	780	3.1	50	520	20	9.33E+11	2500	0.25%	2500	1.55	1.55E+00	3.22E+10	1.29E+10	765-794	150
40-750	40	112	750	2.8	50	520	20	1.05E+12	2500	0.25%	2500	1.74	1.74E+00	2.87E+10	1.15E+10	725-765	150
40-700	40	92	700	2.3	50	520	20	1.31E+12	2500	0.25%	2500	2.19	2.19E+00	2.29E+10	9.14E+09	675-725	60
40-650	40	80	650	2	50	520	20	1.55E+12	2500	0.25%	2500	2.58	2.58E+00	1.94E+10	7.75E+09	625-675	60
40-600	40	68	600	1.7	50	520	20	1.89E+12	2500	0.25%	2500	3.15	3.15E+00	1.59E+10	6.35E+09	575-625	60
40-550	40	60	550	1.5	50	520	20	2.21E+12	2500	0.25%	2500	3.69	3.69E+00	1.36E+10	5.42E+09	525-575	60

50-1064	50	245	1064	4.9	50	530	25	2.89E+11	2500	0.25%	2500	0.48	4.82E-01	1.04E+11	5.18E+10	980-1232	150
50-808	50	145	808	2.9	50	530	25	5.15E+11	2500	0.25%	2500	0.86	8.58E-01	5.83E+10	2.91E+10	750-850	150
50-700	50	110	700	2.2	50	530	25	7.08E+11	2500	0.25%	2500	1.18	1.18E+00	4.24E+10	2.12E+10	650-750	150
50-600	50	100	600	2	50	530	25	7.93E+11	2500	0.25%	2500	1.32	1.32E+00	3.78E+10	1.89E+10	550-650	150

70-750	70	120	750	1.7	50	530	25	3.49E+11	2500	0.25%	2500	0.58	5.81E-01	8.60E+10	4.30E+10	700-800	150
70-650	70	105	650	1.5	50	530	25	4.13E+11	2500	0.25%	2500	0.69	6.88E-01	7.27E+10	3.63E+10	600-700	150
70-550	70	91	550	1.3	50	530	25	4.98E+11	2500	0.25%	2500	0.83	8.30E-01	6.02E+10	3.01E+10	500-600	150

Optical Density >= 50, volume = 1mL

All specs typical. May vary batch to batch. Exact values are measured for each batch

Solution default is 18MEG DI water

Residual Chemicals < 0.1%

Functionalizations

Functionalized gold nanoparticle with Nanopartz™ covalently bonded polymer. Thickness of polymer is 1-2nm.

	Loading Range (nm2)		Charge (mV)
Conugation	Smaller particles	Larger particles	Smaller particles	Larger particles

NiNTA	2	1	-25	-25
HisTag	3	2	-25	-25

Properties, Tech Notes, SDS, Storage and Handling

Details

Composition

These nanoparticles are shipped in 18MEG DI water (default solution) with no measurable residual reactants.

Custom Formulation

Please contact us.

Quantity

This product is available in 1mL volumes and larger.

Introductory Kits

Please contact us.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Functionalization

This product comes with a number of different covalent options.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

This product is known to be noncytotoxic. This product has been sterilized and is biological free.

Sterilization

This product is sterilized.

For post sterilization testing, please choose Sterilization Certification.

For endotoxin purification, choose Endotoxin Purified.

Certifications

Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

UV VIS

Tech Notes, SDS, S/H

TN602 Storage and Handling

TN801 UV VIS to Determine Size and Concentration

SDS Part # C

Sole Source Advantages

Gold Nanorods Characteristics

FAQz, Testimonials, Publications

FAQz

How much thickness does your proprietary ligand add?

Anywhere from 1-2nm.

What about PEG? What is its length?

Click on the link to the PEG Length Technical Note.

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solution you add.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

What is the shelf life if I never open the package and keep refrigerated?

Many years.

Should I store in the refrigerator?

Yes.

How do you size your gold nanoparticles? Does the size include the capping agent?

We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

Why are your small gold nanoparticles colored? I have seen 2nm particles on other website that look as clear as water.

All gold nanoparticles absorb and/or scatter. If you can't see them, then they probably aren't there.

What is OD?

Optical Density (OD) is measured by UV-VIS. An Optical Density OD=1 corresponds to a transmission of 10% through a 1cm cuvette. Optical Density is a nice unit to use since Optical Densities correlate linearly to concentration. So an Optical Density of 1.2 is equal to 1.2 times the concentration of a gold nanoparticle solution that has an Optical Density of 1. We use OD and concentration interchangingly as it is easier to refer to a solution of OD=1 rather than 2.35e12 nanoparticles. For all spheres up to 200nm, OD=1 does refer to 0.05mg/mL.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

How does your polymer bridge compare to PEG?

It is superior in its resistance to salt, pH, and other chemicals.

Testimonials

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

Publications

Enhancing sensitivity of surface plasmon resonance biosensors by functionalized gold nanoparticles: size matters

T Špringer, ML Ermini, B Špačková… - Analytical …, 2014 - ACS Publications

... resistivity, Direct-Q from Millipore). Gold Nanoparticles Spherical AuNPs
covalently functionalized with neutravidin via a 2 nm polymer bridge were
purchased from Nanopartz Inc., USA. The diameters of the gold ...

Physisorption of functionalized gold nanoparticles on AlGaN/GaN high electron mobility transistors for sensing applications

MS Makowski, S Kim, M Gaillard, D Janes… - Applied physics …, 2013 - aip.scitation.org

... used to measure electrical characteristics of physisorbed gold nanoparticles (Au NPs)
functionalized with alkanethiols ... linkers to the gate area of AlGaN/GaN HEMTs include patterning
gold at the ... Also, the effectiveness of the UV functionalization method is offset by the limited ...

[HTML] Enhanced intracellular translocation and biodistribution of gold nanoparticles functionalized with a cell-penetrating peptide (VG-21) from vesicular stomatitis …

PM Tiwari, E Eroglu, SS Bawage, K Vig, ME Miller… - Biomaterials, 2014 - Elsevier

... Carboxyl-polymer coated spherical GNPs (Nanopartz™, Loveland, CO, USA) 13 nm in size ... For
fluorescence microscopy, FITC conjugated VG-21 peptide was used for functionalization. ... GNPs
functionalized with FITC-conjugated VG-21 were used to study internalization of the ...

Wide‐field hyperspectral 3D imaging of functionalized gold nanoparticles targeting cancer cells by reflected light microscopy

S Patskovsky, E Bergeron, D Rioux… - Journal of …, 2015 - Wiley Online Library

... ON) were tethered to AuNPs (50 µg/mL, 100 nm diameter, Nanopartz, A11-100 ... Some examples
of experimental spectral curves for single 100 nm functionalized AuNPs are presented in ... shifted
for about 6 nm that could be explained by AuNPs functionalization and attachment to ...

Size dependence of Au NP-enhanced surface plasmon resonance based on differential phase measurement

S Zeng, X Yu, WC Law, Y Zhang, R Hu, XQ Dinh… - Sensors and Actuators B …, 2013 - Elsevier

... OD = 1.0) with diameters 40 nm, 60 nm, 70 nm and 80 nm were purchased from Nanopartz
Accurate. ... The system was left for a period of 20 min and then rinsed by DI water to confirm the
functionalization. ... A review on functionalized gold nanoparticles for biosensing applications. ...

Mechanistic heteroaggregation of gold nanoparticles in a wide range of solution chemistry

ARMN Afrooz, IA Khan, SM Hussain… - … science & technology, 2013 - ACS Publications

... that can determine aggregation behavior and enumerate aggregation mechanisms of gold
nanospheres (AuNSs) in ... with 10 nm primary particle diameter were procured from Nanopartz
Inc. ... PA-SWNT Preparation To perform surface functionalization with pluronic acid (PA) F127 ...

Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma

T Špringer, J Homola - Analytical and bioanalytical chemistry, 2012 - Springer

... a mixture of carboxy-terminated and hydroxy-terminated thiols were obtained from Nanopartz,
USA, at ... During the functionalization processes described above, the flow rate and the temperature
were set ... BSA was flowed along the surface of an SPR chip functionalized with Ab1 ...

Multiplexed colorimetric detection of Kaposi's sarcoma associated herpesvirus and Bartonella DNA using gold and silver nanoparticles

M Mancuso, L Jiang, E Cesarman, D Erickson - Nanoscale, 2013 - pubs.rsc.org

... Gold and silver nanoparticle functionalization. Gold and silver particles with average diameters
of 15 and 20 nm, respectively, were functionalized using thiol based chemistry using methods
described by others. ... Gold nanoparticles were purchased from Nanopartz (Loveland, CO ...

[HTML] Plasmonic enhancement of dye sensitized solar cells via a tailored size-distribution of chemically functionalized gold nanoparticles

C Andrei, E Lestini, S Crosbie, C de Frein, T O'Reilly… - PLoS …, 2014 - journals.plos.org

... particles with a tailored size distribution and commercially available (spherical) AuNPs Nanopartz,
wt 1.75 ... nm), with optically dispersing TiO 2 particles (Ø ∼190 nm) and functionalized with AuNPs ...
TiO 2 functionalisation with AuNPs) and Figure 4 (after the functionalization) it can ...

Technology

Background

The manufacturing method for these nanoparticles are based on our proprietary method. This family of products have resulted in more than 100 publications and is the cornerstone of our product line. Other covalent polymer bridges are available as well. Please follow the menu to find options from 1 carbon to 30KDa length covalent polymer bridges.

Technology Comparison

	*Nanopartz™ in vitro* Gold Nanoparticles**	PEG Gold Nanoparticles
Stability (salt,pH,chemicals)	High	Medium
Monovalent	Yes	No
Nonspecific binding	Very low	Medium
Sterilization	Yes	No

Features and Benefits

Covalent bonds insure specificity, stability, long shelf life
Buffer Stability - stable from pH 4-9
No sodium azide
No BSA
Polymer coating insures no aggregation in high salts, reduced nonspecific binding
Stable
Well Characterized
Customer can select buffer
Customer can select gold nanoparticle type, size and/or SPR
Loading of all ligands is optimized