PEG Functionalized Gold Nanoparticles - Nanopartz

Nanopartz Gold Nanoparticles, conjugated specifically for in vitro applications, utilize the complete line of Accurate Spherical Gold Nanoparticles, Gold Nanorods, Microgold, and Gold Nanowires conjugated via a PEG Linker to the most popular in vitro terminations including amine, biotin, carboxyl, hydroxyl, methyl, maleimide, nhs, streptavidin, GSH, Protein A and G, and secondary antibodies, with molecular weights from 100Da to 50KDa offered. These particles may also be conjugated to customer defined antibodies, peptides, oligos, and more. Most importantly, Nanopartz™ posseses the proprietary technology to bind to almost any location of the target. For instance, in binding to antibodies, not only does Nanopartz™ conjugate to the antibody, but can to specific sections of the antibody. This ability to covalently conjugate to specific locations ensures the activity of the target is not compromised. These conjugated particles can be used in brightfield, darkfield, fluorescence, and other imaging modes; and ELISA, lateral flow, and other assays. Every batch is completely characterized including size, monodispersity, aggregation, residual chemicals, and concentration. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. In all cases, the loading of the target is determined.

FREE - These products, like all of our products, are BSA Free, Sodium Azide Free, and Glycerol Free

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Product Specifications, Current Stock COAs

Part #CP11 Nanopartz PEG Functionalized Spherical Gold Nanoparticles

Part #	Diameter (nm)	Peak SPR Wavelength (nm)	NPS/ml	Molarity (pM)	Moles	Molar Ext. (M-1cm-1)	Absorption Molar Ext. (M-1cm-1)	Scattering Molar Ext. (M-1cm-1)	Size Dispersity %PDI	Size Accuracy (+/- nm)
CP11-1.8	1.8	n/a	4.25E+16	7.10E+07	7.09E-08	7.05E+05	7.05E+05	0.00E+00	<35%	0.1
CP11-2.2	2.2	n/a	2.33E+16	3.90E+07	3.88E-08	1.29E+06	1.29E+06	0.00E+00	<25%	0.1
CP11-3	3	n/a	9.19E+15	1.50E+07	1.53E-08	3.26E+06	3.26E+06	0.00E+00	<20%	0.1
CP11-4	4	n/a	3.88E+15	6.50E+06	6.46E-09	7.74E+06	7.74E+06	0.00E+00	<20%	1
CP11-5	5	512	1.99E+15	3.30E+06	3.31E-09	1.51E+07	1.51E+07	0.00E+00	<20%	2
CP11-10	10	516	2.48E+14	4.10E+05	4.14E-10	1.21E+08	1.21E+08	0.00E+00	<15%	2
CP11-15	15	518	7.35E+13	1.20E+05	1.23E-10	4.08E+08	4.08E+08	0.00E+00	<15%	2
CP11-20	20	520	3.10E+13	5.20E+04	5.17E-11	9.67E+08	9.67E+08	0.00E+00	<10%	2
CP11-25	25	521	1.59E+13	2.60E+04	2.65E-11	1.89E+09	1.89E+09	0.00E+00	<10%	2
CP11-30	30	523	9.19E+12	1.50E+04	1.53E-11	3.26E+09	3.26E+09	0.00E+00	<6%	2
CP11-35	35	526	5.79E+12	9.60E+03	9.65E-12	5.18E+09	5.18E+09	0.00E+00	<6%	2
CP11-40	40	527	3.88E+12	6.50E+03	6.46E-12	7.74E+09	7.72E+09	2.24E+07	<4%	2
CP11-45	45	529	2.72E+12	4.50E+03	4.54E-12	1.10E+10	1.08E+10	2.03E+08	<4%	2
CP11-50	50	531	1.99E+12	3.30E+03	3.31E-12	1.51E+10	1.45E+10	5.88E+08	<4%	2
CP11-55	55	533	1.49E+12	2.50E+03	2.49E-12	2.01E+10	1.88E+10	1.30E+09	<4%	2
CP11-60	60	536	1.15E+12	1.90E+03	1.91E-12	2.61E+10	2.36E+10	2.48E+09	<4%	2
CP11-65	65	539	9.04E+11	1.50E+03	1.51E-12	3.32E+10	2.89E+10	4.33E+09	<4%	2
CP11-70	70	542	7.23E+11	1.20E+03	1.21E-12	4.15E+10	3.44E+10	7.09E+09	<4%	2
CP11-75	75	545	5.88E+11	9.80E+02	9.80E-13	5.10E+10	4.00E+10	1.10E+10	<4%	2
CP11-80	80	549	4.85E+11	8.10E+02	8.08E-13	6.19E+10	4.54E+10	1.65E+10	<4%	2
CP11-85	85	553	4.04E+11	6.70E+02	6.73E-13	7.42E+10	5.03E+10	2.39E+10	<4%	2
CP11-90	90	558	3.40E+11	5.70E+02	5.67E-13	8.81E+10	5.44E+10	3.37E+10	<4%	2
CP11-95	95	563	2.89E+11	4.80E+02	4.82E-13	1.04E+11	5.72E+10	4.65E+10	<4%	2
CP11-100	100	569	2.48E+11	4.10E+02	4.14E-13	1.21E+11	5.82E+10	6.27E+10	<4%	2
CP11-150	150	612	7.35E+10	1.20E+02	1.23E-13	4.08E+11	0.00E+00	4.08E+11	<4%	10
CP11-200	200	n/a	3.10E+10	5.20E+01	5.17E-14	9.67E+11	0.00E+00	9.67E+11	<4%	10
CP11-500	500	n/a	1.99E+09	3.30E+00	3.31E-15	1.51E+13	0.00E+00	1.51E+13	<4%	50
CP11-1000	1000	n/a	2.48E+08	4.10E-01	4.14E-16	1.21E+14	0.00E+00	1.21E+14	<4%	100
CP11-1500	1500	n/a	7.35E+07	1.20E-01	1.23E-16	4.08E+14	0.00E+00	4.08E+14	<4%	100

Optical Density >= 50, 5 for sizes >=500nm

Wt conc = 2.5mg/mL, 1mL volume typical

Wt % = 0.25%

ppm = 2500

%PDI = Std Dev/Size

See Tech Note TN801 for definition of terms and method of analysis

All specs typical. May vary batch to batch. Exact values are measured for each batch

Shape monodispersity (% spheres) > 99.9%

Solution default is 18MEG DI water

Residual Chemicals < 0.1%

Part #CP12 Nanopartz PEG Functionalized Gold Nanorods

Part #	Diameter (nm)	Length (nm)	Peak SPR Wave (nm)	Aspect Ratio	OD SPR (AU)	Peak LSPR Wave (nm)	OD LSPR (AU)	Nanorods /mL	Wt. conc (ug/ml)	Wt. %	PPM	Molarity (nM)	Picomoles	SPR Molar Ext. (M-1cm-1)	LSPR Molar Ext. (M-1cm-1)	Peak SPR (nm)	SPR Linewidth 80% (nm)
CP12-5-808	5	21	808	4.2	50	510	10	2.39E+14	1750	0.18%	1750	398.72	3.99E+02	1.25E+08	2.51E+07	794-829	100
CP12-5-780	5	19	780	3.8	50	510	10	2.67E+14	1750	0.18%	1750	444.72	4.45E+02	1.12E+08	2.25E+07	765-794	100
CP12-5-700	5	15	700	3	50	510	10	3.47E+14	1750	0.18%	1750	578.14	5.78E+02	8.65E+07	1.73E+07	675-725	100

CP12-10-2100	10	175	2100	17.5	50	510	10	6.73E+12	1750	0.18%	1750	11.21	1.12E+01	4.46E+09	8.92E+08	1900-2300	300
CP12-10-1400	10	102	1400	10.2	50	510	10	1.17E+13	1750	0.18%	1750	19.47	1.95E+01	2.57E+09	5.14E+08	1300-1500	150
CP12-10-1200	10	81	1200	8.1	50	510	10	1.48E+13	1750	0.18%	1750	24.67	2.47E+01	2.03E+09	4.05E+08	1132-1300	150
CP12-10-1064	10	67	1064	6.7	50	510	10	1.81E+13	1750	0.18%	1750	30.13	3.01E+01	1.66E+09	3.32E+08	1022-1132	150
CP12-10-980	10	59	980	5.9	50	510	10	2.09E+13	1750	0.18%	1750	34.91	3.49E+01	1.43E+09	2.86E+08	965-1022	150
CP12-10-950	10	55	950	5.5	50	510	10	2.22E+13	1750	0.18%	1750	37	3.70E+01	1.35E+09	2.70E+08	925-965	150
CP12-10-900	10	50	900	5	50	510	10	2.47E+13	1750	0.18%	1750	41.11	4.11E+01	1.22E+09	2.43E+08	875-925	150
CP12-10-850	10	45	850	4.5	50	510	10	2.78E+13	1750	0.18%	1750	46.25	4.63E+01	1.08E+09	2.16E+08	829-875	100
CP12-10-808	10	41	808	4.1	50	510	10	3.10E+13	1750	0.18%	1750	51.68	5.17E+01	9.68E+08	1.94E+08	794-829	75
CP12-10-780	10	38	780	3.8	50	510	10	3.36E+13	1750	0.18%	1750	56.06	5.61E+01	8.92E+08	1.78E+08	765-794	65
CP12-10-750	10	35	750	3.5	50	510	10	3.70E+13	1750	0.18%	1750	61.67	6.17E+01	8.11E+08	1.62E+08	725-765	50
CP12-10-700	10	29	700	2.9	50	510	10	4.44E+13	1750	0.18%	1750	74	7.40E+01	6.76E+08	1.35E+08	675-725	40
CP12-10-650	10	24	650	2.4	50	510	10	5.55E+13	1750	0.18%	1750	92.5	9.25E+01	5.41E+08	1.08E+08	625-675	40
CP12-10-600	10	19	600	1.9	50	510	10	7.40E+13	1750	0.18%	1750	123.34	1.23E+02	4.05E+08	8.11E+07	575-625	40

CP12-25-1400	25	245	1400	9.8	50	514	15	1.12E+12	2500	0.25%	2500	1.86	1.86E+00	2.69E+10	8.06E+09	1232-1500	150
CP12-25-1064	25	137	1064	5.5	50	514	15	2.05E+12	2500	0.25%	2500	3.42	3.42E+00	1.46E+10	4.38E+09	1022-1232	150
CP12-25-980	25	119	980	4.8	50	514	15	2.39E+12	2500	0.25%	2500	3.98	3.98E+00	1.26E+10	3.77E+09	965-1022	150
CP12-25-950	25	102	950	4.1	50	514	15	2.82E+12	2500	0.25%	2500	4.7	4.70E+00	1.06E+10	3.19E+09	925-965	150
CP12-25-900	25	96	900	3.8	50	514	15	3.01E+12	2500	0.25%	2500	5.02	5.02E+00	9.95E+09	2.99E+09	875-925	150
CP12-25-850	25	93	850	3.7	50	514	15	3.12E+12	2500	0.25%	2500	5.2	5.20E+00	9.61E+09	2.88E+09	829-875	150
CP12-25-808	25	90	808	3.6	50	514	15	3.24E+12	2500	0.25%	2500	5.39	5.39E+00	9.27E+09	2.78E+09	794-829	150
CP12-25-780	25	87	780	3.5	50	514	15	3.36E+12	2500	0.25%	2500	5.6	5.60E+00	8.93E+09	2.68E+09	765-794	150
CP12-25-750	25	85	750	3.4	50	514	15	3.45E+12	2500	0.25%	2500	5.75	5.75E+00	8.70E+09	2.61E+09	725-765	65
CP12-25-700	25	75	700	3	50	514	15	3.96E+12	2500	0.25%	2500	6.61	6.61E+00	7.57E+09	2.27E+09	675-725	60
CP12-25-650	25	71	650	2.8	50	514	15	4.22E+12	2500	0.25%	2500	7.03	7.03E+00	7.11E+09	2.13E+09	625-675	60
CP12-25-600	25	57	600	2.3	50	514	15	5.43E+12	2500	0.25%	2500	9.05	9.05E+00	5.52E+09	1.66E+09	575-625	60
CP12-25-550	25	34	550	1.4	50	514	15	1.01E+13	2500	0.25%	2500	16.91	1.69E+01	2.96E+09	8.87E+08	525-575	60

CP12-40-1064	40	208	850	5.2	50	520	20	5.30E+11	2500	0.25%	2500	0.88	8.84E-01	5.66E+10	2.26E+10	1022-1232	150
CP12-40-980	40	180	850	4.5	50	520	20	6.19E+11	2500	0.25%	2500	1.03	1.03E+00	4.84E+10	1.94E+10	875-1022	150
CP12-40-850	40	148	850	3.7	50	520	20	7.67E+11	2500	0.25%	2500	1.28	1.28E+00	3.91E+10	1.57E+10	829-875	150
CP12-40-808	40	134	808	3.4	50	520	20	8.56E+11	2500	0.25%	2500	1.43	1.43E+00	3.51E+10	1.40E+10	794-829	150
CP12-40-780	40	124	780	3.1	50	520	20	9.33E+11	2500	0.25%	2500	1.55	1.55E+00	3.22E+10	1.29E+10	765-794	150
CP12-40-750	40	112	750	2.8	50	520	20	1.05E+12	2500	0.25%	2500	1.74	1.74E+00	2.87E+10	1.15E+10	725-765	150
CP12-40-700	40	92	700	2.3	50	520	20	1.31E+12	2500	0.25%	2500	2.19	2.19E+00	2.29E+10	9.14E+09	675-725	60
CP12-40-650	40	80	650	2	50	520	20	1.55E+12	2500	0.25%	2500	2.58	2.58E+00	1.94E+10	7.75E+09	625-675	60
CP12-40-600	40	68	600	1.7	50	520	20	1.89E+12	2500	0.25%	2500	3.15	3.15E+00	1.59E+10	6.35E+09	575-625	60
CP12-40-550	40	60	550	1.5	50	520	20	2.21E+12	2500	0.25%	2500	3.69	3.69E+00	1.36E+10	5.42E+09	525-575	60

CP12-50-1064	50	245	1064	4.9	50	530	25	2.89E+11	2500	0.25%	2500	0.48	4.82E-01	1.04E+11	5.18E+10	980-1232	150
CP12-50-808	50	145	808	2.9	50	530	25	5.15E+11	2500	0.25%	2500	0.86	8.58E-01	5.83E+10	2.91E+10	750-850	150
CP12-50-700	50	110	700	2.2	50	530	25	7.08E+11	2500	0.25%	2500	1.18	1.18E+00	4.24E+10	2.12E+10	650-750	150
CP12-50-600	50	100	600	2	50	530	25	7.93E+11	2500	0.25%	2500	1.32	1.32E+00	3.78E+10	1.89E+10	550-650	150

CP12-70-750	70	120	750	1.7	50	530	25	3.49E+11	2500	0.25%	2500	0.58	5.81E-01	8.60E+10	4.30E+10	700-800	150
CP12-70-650	70	105	650	1.5	50	530	25	4.13E+11	2500	0.25%	2500	0.69	6.88E-01	7.27E+10	3.63E+10	600-700	150
CP12-70-550	70	91	550	1.3	50	530	25	4.98E+11	2500	0.25%	2500	0.83	8.30E-01	6.02E+10	3.01E+10	500-600	150

Optical Density >= 50, volume = 1mL

All specs typical. May vary batch to batch. Exact values are measured for each batch

Solution default is 18MEG DI water

Residual Chemicals < 0.1%

Part #CP13 Nanopartz PEG Functionalized Microgold

Part #	Diameter (nm)	Length (nm)	Aspect Ratio	Peak SPR Wave (nm)	SPR OD (1cm)	Microgold /mL	moles	Wt. conc (ug/ml)	Wt. %	PPM	Molarity (pM)	SPR Molar Ext. (M-1cm-1)
CP13-500	75	500	6.7	510	0.25	6.18E+09	1.03E-14	250	0.03%	250	10.3	2.43E+07
CP13-1000	100	1000	10	510	0.25	1.71E+09	2.85E-15	250	0.03%	250	2.85	8.78E+07

SPR = Transverse SPR peak

Longitudinal has no SPR peak

Shape monodispersity (% rods) > 95%

Size variation +/-10% (both dimensions)

Part #CP14 Nanopartz PEG Functionalized Gold Nanowires

Part #	Diameter (nm)	Length (nm)	Aspect Ratio	Peak SPR Wave (nm)	Nanowires Vol (nm3)	Nanowires /mL	Wt. conc (ug/ml)	Wt. %	PPM	Molarity (pM)	SPR Molar Ext. (M-1cm-1)
CP14-1000	75	1000	13	510	4.31E+06	3.01E+09	250	0.03%	250	5.02	9.96E+06
CP14-2000	75	2000	27	510	8.72E+06	1.49E+09	250	0.03%	250	2.48	2.02E+07
CP14-4000	75	4000	53	510	1.76E+07	7.39E+08	250	0.03%	250	1.23	4.06E+07
CP14-6000	75	6000	80	510	2.64E+07	4.91E+08	250	0.03%	250	0.82	6.10E+07
CP14-10000	75	10000	133	510	4.40E+07	2.94E+08	250	0.03%	250	0.49	1.02E+08
CP14-20000	100	20000	200	510	1.57E+08	8.27E+07	250	0.03%	250	0.14	3.63E+08
CP14-40000	140	40000	286	510	6.15E+08	2.11E+07	250	0.03%	250	0.04	1.42E+09

SPR = Transverse SPR peak

Longitudinal has no SPR peak

Shape monodispersity (% rods) > 95%

Size variation +/-10% (both dimensions)

Functionalizations

Functionalized gold nanoparticle with PEG polymer

	Loading Range (nm2)		Charge (mV)
	Smaller particles	Larger particles	Smaller particles	Larger particles

Amine	3	1	-20	20
Biotin	2	1	-25	-15
Carboxyl	3	1	-38	-45
Methyl	3	1	-27	-12
Streptavidin	0.01	0.01	-25	-10
Protein A	0.04	0.04	-25	-10
Maleimide	3	1	-27	-12
Hydroxyl	3	1	-27	-12
Secondary IgG	0.02	0.02	0.02	0.02

Properties, Tech Notes, SDS, Storage and Handling

Details

Composition

These nanoparticles are shipped in 18MEG DI water (default solution) with no measurable residual reactants.

Custom Formulation

Please contact us.

Quantity

This product is available in 1mL volumes and larger.

Introductory Kits

Please contact us.

Delivery

Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.

Functionalization

This product comes with a number of different covalent options.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.

Toxicity

This product is known to be noncytotoxic. This product has been sterilized and is biological free.

Sterilization

This product is sterilized.

For post sterilization testing, please choose Sterilization Certification.

For endotoxin purification, choose Endotoxin Purified.

Certifications

Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

UV VIS

Tech Notes, SDS, S/H

TN602 Storage and Handling

TN801 UV VIS to Determine Size and Concentration

SDS Part # C

Sole Source Advantages

Gold Nanorods Characteristics

FAQz, Testimonials, Publications

FAQz

How much thickness does PEG add?

Go here: PEG Tech Note

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solution you add.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

Can I suspend in silicon oil?

What is the shelf life if I never open the package and keep refrigerated?

Many years.

Should I store in the refrigerator?

Yes.

How do you size your gold nanoparticles? Does the size include the capping agent?

We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.

Do you really provide a TEM for my specific lot?

Yes, and not just for 5-10 particles, rather 50-100 are standard.

Why are your small gold nanoparticles colored? I have seen 2nm particles on other website that look as clear as water.

All gold nanoparticles absorb and/or scatter. If you can't see them, then they probably aren't there.

What is OD?

Optical Density (OD) is measured by UV-VIS. An Optical Density OD=1 corresponds to a transmission of 10% through a 1cm cuvette. Optical Density is a nice unit to use since Optical Densities correlate linearly to concentration. So an Optical Density of 1.2 is equal to 1.2 times the concentration of a gold nanoparticle solution that has an Optical Density of 1. We use OD and concentration interchangingly as it is easier to refer to a solution of OD=1 rather than 2.35e12 nanoparticles. For all spheres up to 200nm, OD=1 does refer to 0.05mg/mL.

What is PDI?

PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.

How does your polymer bridge compare to PEG?

It is superior in its resistance to salt, pH, and other chemicals. They are found here: Nanopartz In Vitro Functionalized Gold Nanoparticles

Testimonials

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

Publications

Enhancing sensitivity of surface plasmon resonance biosensors by functionalized gold nanoparticles: size matters

T Špringer, ML Ermini, B Špačková… - Analytical …, 2014 - ACS Publications

... resistivity, Direct-Q from Millipore). Gold Nanoparticles Spherical AuNPs
covalently functionalized with neutravidin via a 2 nm polymer bridge were
purchased from Nanopartz Inc., USA. The diameters of the gold ...

Physisorption of functionalized gold nanoparticles on AlGaN/GaN high electron mobility transistors for sensing applications

MS Makowski, S Kim, M Gaillard, D Janes… - Applied physics …, 2013 - aip.scitation.org

... used to measure electrical characteristics of physisorbed gold nanoparticles (Au NPs)
functionalized with alkanethiols ... linkers to the gate area of AlGaN/GaN HEMTs include patterning
gold at the ... Also, the effectiveness of the UV functionalization method is offset by the limited ...

[HTML] Enhanced intracellular translocation and biodistribution of gold nanoparticles functionalized with a cell-penetrating peptide (VG-21) from vesicular stomatitis …

PM Tiwari, E Eroglu, SS Bawage, K Vig, ME Miller… - Biomaterials, 2014 - Elsevier

... Carboxyl-polymer coated spherical GNPs (Nanopartz™, Loveland, CO, USA) 13 nm in size ... For
fluorescence microscopy, FITC conjugated VG-21 peptide was used for functionalization. ... GNPs
functionalized with FITC-conjugated VG-21 were used to study internalization of the ...

Wide‐field hyperspectral 3D imaging of functionalized gold nanoparticles targeting cancer cells by reflected light microscopy

S Patskovsky, E Bergeron, D Rioux… - Journal of …, 2015 - Wiley Online Library

... ON) were tethered to AuNPs (50 µg/mL, 100 nm diameter, Nanopartz, A11-100 ... Some examples
of experimental spectral curves for single 100 nm functionalized AuNPs are presented in ... shifted
for about 6 nm that could be explained by AuNPs functionalization and attachment to ...

Size dependence of Au NP-enhanced surface plasmon resonance based on differential phase measurement

S Zeng, X Yu, WC Law, Y Zhang, R Hu, XQ Dinh… - Sensors and Actuators B …, 2013 - Elsevier

... OD = 1.0) with diameters 40 nm, 60 nm, 70 nm and 80 nm were purchased from Nanopartz
Accurate. ... The system was left for a period of 20 min and then rinsed by DI water to confirm the
functionalization. ... A review on functionalized gold nanoparticles for biosensing applications. ...

Mechanistic heteroaggregation of gold nanoparticles in a wide range of solution chemistry

ARMN Afrooz, IA Khan, SM Hussain… - … science & technology, 2013 - ACS Publications

... that can determine aggregation behavior and enumerate aggregation mechanisms of gold
nanospheres (AuNSs) in ... with 10 nm primary particle diameter were procured from Nanopartz
Inc. ... PA-SWNT Preparation To perform surface functionalization with pluronic acid (PA) F127 ...

Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma

T Špringer, J Homola - Analytical and bioanalytical chemistry, 2012 - Springer

... a mixture of carboxy-terminated and hydroxy-terminated thiols were obtained from Nanopartz,
USA, at ... During the functionalization processes described above, the flow rate and the temperature
were set ... BSA was flowed along the surface of an SPR chip functionalized with Ab1 ...

Multiplexed colorimetric detection of Kaposi's sarcoma associated herpesvirus and Bartonella DNA using gold and silver nanoparticles

M Mancuso, L Jiang, E Cesarman, D Erickson - Nanoscale, 2013 - pubs.rsc.org

... Gold and silver nanoparticle functionalization. Gold and silver particles with average diameters
of 15 and 20 nm, respectively, were functionalized using thiol based chemistry using methods
described by others. ... Gold nanoparticles were purchased from Nanopartz (Loveland, CO ...

[HTML] Plasmonic enhancement of dye sensitized solar cells via a tailored size-distribution of chemically functionalized gold nanoparticles

C Andrei, E Lestini, S Crosbie, C de Frein, T O'Reilly… - PLoS …, 2014 - journals.plos.org

... particles with a tailored size distribution and commercially available (spherical) AuNPs Nanopartz,
wt 1.75 ... nm), with optically dispersing TiO 2 particles (Ø ∼190 nm) and functionalized with AuNPs ...
TiO 2 functionalisation with AuNPs) and Figure 4 (after the functionalization) it can ...

Technology

Background

The manufacturing method for these nanoparticles are based on our proprietary method. Standard PEGylation can result in poor PEG density leading to poor salt and pH resistance and loading. We use chemistry, temperature, pH, concentration, and physical methods to optimize PEG densities.

Technology Comparison

	*Nanopartz™ in vitro* Gold Nanoparticles**	PEG Gold Nanoparticles
Stability (salt,pH,chemicals)	High	Medium
Monovalent	Yes	No
Nonspecific binding	Very low	Medium
Sterilization	Yes	No

Features and Benefits

Covalent bonds insure specificity, stability, long shelf life
Buffer Stability - stable from pH 5-8
No sodium azide
No BSA
Polymer coating insures no aggregation in high salts, reduced nonspecific binding
Stable
Well Characterized
Customer can select buffer
Customer can select gold nanoparticle type, size and/or SPR
Loading of all ligands is optimized