Nanopartz™ Gold Nanoparticles for Zero Order Diagnostics

 

Nanopartz™ covalently bonded Zero Order Construct.   Conjugations (Ordering Abbreviation) Amine TA Biotin TB Carboxyl TC Methyl TM Neutravidin TN NHS TNHS Streptavidin TS Secondary antibodies

 

Composition

Available with the following active groups. Methyl acts as a free acid form to be used as a non-reactive negative control.

1. Amine
2. Biotin
3. Carboxyl
4. Neutravidin
5. Streptavidin
6. Azide

Secondary Antibody Conjugates - polyclonal provide species-specific detection of the entire target IgG class (heavy and light chains, H+L). Reagents, labeling (PF/protein ratio and purificaiton methods are optimized to provide the highest possible binding function and overall fluorescence intensity. All conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use.

1. Goat Anti-Human IgG
2. Goat Anti-Mouse IgG
3. Goat Anti-Rabitt IgG
4. Goat Anti-Human IgA
5. Goat Anti-Human IgM

Quantity

This product is available in 50 OD-mL and 250 OD-mL quantities, 2.5mg and 12.5mg gold, respectively. Volume is 1mL. Buffer is customer determined.

Delivery

All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays.

Shelf Life/Storage Temperature

This product is guaranteed for six months and should be stored at 4°C after opening. Care must be taken to only use sterile glassware when working with this product.

Sterilization/Endotoxin

Sterilization and Endoxtoin Free Options are available.

Certifications

This product is manufactured using our audited ISO 9000/2001 quality control system. Every order comes with a Certification of Analysis. We use NIST traceable:

UV-VIS (Agilent 8453) for extinction and concentration measurements

NIR (Cary 500) for NIR extinction and concentration measurements

DLS (Malvern Nano ZS) for zeta potential measurement

ICP-MS (Varian 820-MS) for gold mass measurements

TEM (Phillips CM-100 100KV) for sizing

These gold nanoparticles exhibit extinctions from 500nm to 1064nm, perfect for use in UV-VIS analytical systems. These gold nanoparticles come with a very short length covalent conjugation, specifically suited for zero order diagnostics as it is a well known property that the extinction of gold nanoparticles is very sensitive to the local index of refraction. The covalent attachment ensures minimal non specific binding. The customer has a choice for radiation sterilization (no sodium azide is used), certification and endotoxin free products.

Nanopartz™ Gold Nanoparticles for Zero Order Citrate spherical gold nanoparticles with adsorbed conjugates Stability (salt,pH,chemicals) High Low Scattering linewidth Narrow (monodisperse) Broad (polydisperse) Multilabeling Yes (500nm-2100nm) Limited (520nm-600nm) Nonspecific binding Very low High pH and salt resistance High Medium Sterilization Radiation Sodium Azide Site specific antibody conjugation Yes No

• Extremely stable. Shelf life is greater than six months.
• Covalent bonds.
• High anisotropy.
• No cytotoxicity.
• Nanorod to sphere shape changing on high energy pulsed lasers.
• Bright Fluorescence - intense emission provides superior sensitivity and requires less conjugate.
• Narrow Emission spectra - covalent bonds ensure no bleed through.
• Excellent photostability - exceptional resistance to photobleaching enables fluorescence imaging uner the most demanding conditions.
• Buffer Stability - stable from pH 4-9.
• Instrument cability - excitation and emission spectra correspond with filter sets and laser setting of all popular fluorescence instrumentation.

• Customer can focus on research and not on Nanopartz™ expertise
• Quick turnaround

How sensitive is the product?

That depends on a number of factors including the size and shape of the gold nanoparticle and the size of the target protein. In general, nanorods are more sensitive than spheres (shifts in aspect ratio result in a greater SPR change than a change in diameter for spheres), and larger particles are more sensitive than smaller ones. Also, a system targeting larger proteins will be more sensitive than one targeting smaller proteins.

Another very important variable is whether the system is a homogeneous or heterogeneous system. In general, a homogeneous system will measure many particles simultaneously (normally through a path length). A heterogenous system can be designed to look at individual, fixed gold nanoparticles on a slide (for example). Consequently, the fact that single particle statistics are more sensitive than bulk particle statistics, a heterogeneous system will be more sensitive.

Given these parameters, publications have shown attomolar detection for nanorods using a biotin/streptavidin system in a heterogenous system on a glass slide. A good example of this is shown in Nucz et al. "Label-Free Plasmonic Detection of Biomolecular Binding by a Single Gold Nanorod" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003601/ .

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University