Gold Nanoparticles for Biosensing and In Vitro Diagnostics
Gold nanoparticles have revolutionized the field of biosensing, providing an advanced platform for highly sensitive and specific detection of biomolecules. Among the various types of nanomaterials available, gold nanoparticles—particularly gold nanorods—have emerged as the most effective due to their unique optical properties. One of the key mechanisms that make gold nanorods indispensable in biosensing is Localized Surface Plasmon Resonance (LSPR) shifting, which allows for real-time, label-free detection of biomolecular interactions.
“It’s indeed a useful product that could find its way to a number of applications and customers including plasmonic bio-sensing experiments ”
Radwanul Hasan
Siddique Research Group Lead at Samsung R&D
Pasadena, California
SPR shifting for a gold nanorod due to analyte binding changing the index of refraction.
What is LSPR Sensing?
Localized Surface Plasmon Resonance (LSPR) is an optical phenomenon that occurs when conduction electrons on the surface of gold nanoparticles oscillate in response to incident light. This creates a strong resonance effect that is highly sensitive to changes in the surrounding environment. Please go here (How Refractive Index Influences Gold Nanoparticle SPR) for our technical note on SPR shifting with the change in localized index of refraction. When biomolecules bind to the surface of gold nanoparticles, the local refractive index changes, leading to a measurable LSPR shift—a spectral shift in the peak absorption or scattering of light. By monitoring this shift, researchers can detect molecular interactions, quantify biomolecules, and even study binding kinetics in real time without the need for fluorescent labels or additional reagents.
LSPR-based biosensing has become a cornerstone of modern in vitro diagnostics due to its high specificity, real-time detection capability, and ability to work in complex biological samples without signal interference.
Advantages of Gold Nanorods for Biosensing
Gold nanorods offer distinct advantages over spherical gold nanoparticles when used in LSPR biosensing. The elongated shape of nanorods provides tunable plasmonic properties, allowing for precise control of resonance wavelengths from the visible to the near-infrared (NIR) region. This tunability enables:
- Enhanced Sensitivity – Gold nanorods exhibit sharper and more intense LSPR peaks compared to spherical nanoparticles, making even minor molecular interactions detectable.
- Broader Spectral Range – The longitudinal plasmon resonance of nanorods can be engineered to match the optimal wavelength for different detection platforms.
- Higher Stability – The larger aspect ratio of nanorods leads to increased stability in biological environments, reducing signal drift and improving assay reproducibility.
- Real-Time, Label-Free Detection – Unlike fluorescence-based methods, LSPR biosensing with gold nanorods does not require external labels or dyes, minimizing sample preparation steps and reducing potential interference.
- Coupled Longitudinal and Axial SPR – Unlike spherical nanoparticles, gold nanorods have two distinct surface plasmon resonances: the longitudinal (along the long axis) and the axial (across the short axis). These two resonances are interdependent, meaning any molecular binding event affecting one resonance will also influence the other. This coupling effect enhances biosensing accuracy by providing a more amplified and reliable LSPR shift, ultimately leading to improved detection sensitivity.
Product Description: Precision-Engineered Gold Nanorods for Biosensing
At Nanopartz, we have designed our gold nanorods with a highly specialized short bridge ligand structure, optimized for LSPR biosensing in biological environments. This short bridge ligand connects the gold surface to the active terminal group, which serves as the binding moiety for analyte conjugation.
Key Features of Our Gold Nanorod Design:
✔ Salt-Resistant Surface Chemistry – The short bridge ligand structure provides exceptional stability in PBS and other high-salt biological buffers, preventing nanoparticle aggregation and ensuring consistent LSPR performance.
✔ Maximized SPR Shift for Enhanced Sensitivity – The ultra-short bridge ligand minimizes steric hindrance, allowing analytes to interact closer to the gold nanorod surface, resulting in a stronger LSPR shift. This enhances detection sensitivity, even for low-concentration biomolecules.
✔ Coupled Longitudinal and Axial SPR for Improved Detection – Unlike spherical nanoparticles, the two distinct plasmon modes in gold nanorods are interconnected. This coupled SPR effect ensures that even small biomolecular interactions lead to significant LSPR shifts, improving both detection accuracy and signal reliability.
✔ High Conjugation Efficiency – The active terminal group is designed for efficient binding to a wide range of biomolecules, including antibodies, proteins, nucleic acids, and small molecules, providing reliable and reproducible biosensing assays.
✔ Optimized for Label-Free Biosensing – Our gold nanorods are engineered for direct interaction with target analytes, eliminating the need for secondary labels or fluorescent tags while maintaining high specificity.
Applications in Biosensing and In Vitro Diagnostics
The ability of gold nanorods to detect molecular binding events with high sensitivity makes them ideal for in vitro diagnostics across a wide range of applications:
- Point-of-Care Testing (POCT) – LSPR biosensors using gold nanorods provide rapid, on-site detection of biomarkers, enabling early disease diagnosis without the need for specialized laboratory equipment.
- Lateral Flow Assays – The colorimetric changes induced by LSPR shifts in gold nanorods improve the accuracy and reliability of paper-based diagnostic tests.
- Electrochemical Biosensors – Gold nanorods enhance the electrochemical signal response in biosensors, allowing for ultra-sensitive detection of proteins, nucleic acids, and pathogens.
- Drug Discovery & Biomolecular Research – LSPR-based assays are widely used to study protein-protein interactions, antibody binding, and drug-receptor interactions in pharmaceutical research.
Why Choose Nanopartz® Gold Nanoparticles for LSPR Biosensing?
Nanopartz gold nanoparticles are precision-engineered to provide maximum sensitivity and stability in LSPR biosensing applications. With over 14 years of experience and more than 7,000 customers worldwide, Nanopartz has established itself as a leader in advanced gold nanoparticle solutions. Our gold nanorods are available in a range of aspect ratios, allowing researchers to customize LSPR wavelengths for their specific detection needs.
Additionally, Nanopartz gold nanoparticles are:
✔ Highly Monodisperse – Ensuring reproducible and consistent LSPR shifts
✔ Surface-Functionalized – Available with carboxyl, amine, and thiol modifications for easy biomolecule conjugation
✔ Optimized for Stability – Designed to maintain structural integrity in physiological conditions
Part # |
Diameter (nm) |
Peak SPR Wavelength (nm) |
NPS/ml |
Molarity (pM) |
Moles |
Molar Ext. (M-1cm-1) |
Absorption Molar Ext. (M-1cm-1) |
Scattering Molar Ext. (M-1cm-1) |
Size Dispersity %PDI |
Size Accuracy (+/- nm) |
1.8 |
1.8 |
n/a |
4.25E+16 |
7.10E+07 |
7.09E-08 |
7.05E+05 |
7.05E+05 |
0.00E+00 |
<35% |
0.1 |
2.2 |
2.2 |
n/a |
2.33E+16 |
3.90E+07 |
3.88E-08 |
1.29E+06 |
1.29E+06 |
0.00E+00 |
<25% |
0.1 |
3 |
3 |
n/a |
9.19E+15 |
1.50E+07 |
1.53E-08 |
3.26E+06 |
3.26E+06 |
0.00E+00 |
<20% |
0.1 |
4 |
4 |
n/a |
3.88E+15 |
6.50E+06 |
6.46E-09 |
7.74E+06 |
7.74E+06 |
0.00E+00 |
<20% |
1 |
5 |
5 |
512 |
1.99E+15 |
3.30E+06 |
3.31E-09 |
1.51E+07 |
1.51E+07 |
0.00E+00 |
<20% |
2 |
10 |
10 |
516 |
2.48E+14 |
4.10E+05 |
4.14E-10 |
1.21E+08 |
1.21E+08 |
0.00E+00 |
<15% |
2 |
15 |
15 |
518 |
7.35E+13 |
1.20E+05 |
1.23E-10 |
4.08E+08 |
4.08E+08 |
0.00E+00 |
<15% |
2 |
20 |
20 |
520 |
3.10E+13 |
5.20E+04 |
5.17E-11 |
9.67E+08 |
9.67E+08 |
0.00E+00 |
<10% |
2 |
25 |
25 |
521 |
1.59E+13 |
2.60E+04 |
2.65E-11 |
1.89E+09 |
1.89E+09 |
0.00E+00 |
<10% |
2 |
30 |
30 |
523 |
9.19E+12 |
1.50E+04 |
1.53E-11 |
3.26E+09 |
3.26E+09 |
0.00E+00 |
<6% |
2 |
35 |
35 |
526 |
5.79E+12 |
9.60E+03 |
9.65E-12 |
5.18E+09 |
5.18E+09 |
0.00E+00 |
<6% |
2 |
40 |
40 |
527 |
3.88E+12 |
6.50E+03 |
6.46E-12 |
7.74E+09 |
7.72E+09 |
2.24E+07 |
<4% |
2 |
45 |
45 |
529 |
2.72E+12 |
4.50E+03 |
4.54E-12 |
1.10E+10 |
1.08E+10 |
2.03E+08 |
<4% |
2 |
50 |
50 |
531 |
1.99E+12 |
3.30E+03 |
3.31E-12 |
1.51E+10 |
1.45E+10 |
5.88E+08 |
<4% |
2 |
55 |
55 |
533 |
1.49E+12 |
2.50E+03 |
2.49E-12 |
2.01E+10 |
1.88E+10 |
1.30E+09 |
<4% |
2 |
60 |
60 |
536 |
1.15E+12 |
1.90E+03 |
1.91E-12 |
2.61E+10 |
2.36E+10 |
2.48E+09 |
<4% |
2 |
65 |
65 |
539 |
9.04E+11 |
1.50E+03 |
1.51E-12 |
3.32E+10 |
2.89E+10 |
4.33E+09 |
<4% |
2 |
70 |
70 |
542 |
7.23E+11 |
1.20E+03 |
1.21E-12 |
4.15E+10 |
3.44E+10 |
7.09E+09 |
<4% |
2 |
75 |
75 |
545 |
5.88E+11 |
9.80E+02 |
9.80E-13 |
5.10E+10 |
4.00E+10 |
1.10E+10 |
<4% |
2 |
80 |
80 |
549 |
4.85E+11 |
8.10E+02 |
8.08E-13 |
6.19E+10 |
4.54E+10 |
1.65E+10 |
<4% |
2 |
85 |
85 |
553 |
4.04E+11 |
6.70E+02 |
6.73E-13 |
7.42E+10 |
5.03E+10 |
2.39E+10 |
<4% |
2 |
90 |
90 |
558 |
3.40E+11 |
5.70E+02 |
5.67E-13 |
8.81E+10 |
5.44E+10 |
3.37E+10 |
<4% |
2 |
95 |
95 |
563 |
2.89E+11 |
4.80E+02 |
4.82E-13 |
1.04E+11 |
5.72E+10 |
4.65E+10 |
<4% |
2 |
100 |
100 |
569 |
2.48E+11 |
4.10E+02 |
4.14E-13 |
1.21E+11 |
5.82E+10 |
6.27E+10 |
<4% |
2 |
150 |
150 |
612 |
7.35E+10 |
1.20E+02 |
1.23E-13 |
4.08E+11 |
0.00E+00 |
4.08E+11 |
<4% |
10 |
200 |
200 |
n/a |
3.10E+10 |
5.20E+01 |
5.17E-14 |
9.67E+11 |
0.00E+00 |
9.67E+11 |
<4% |
10 |
500 |
500 |
n/a |
1.99E+09 |
3.30E+00 |
3.31E-15 |
1.51E+13 |
0.00E+00 |
1.51E+13 |
<4% |
50 |
1000 |
1000 |
n/a |
2.48E+08 |
4.10E-01 |
4.14E-16 |
1.21E+14 |
0.00E+00 |
1.21E+14 |
<4% |
100 |
1500 |
1500 |
n/a |
7.35E+07 |
1.20E-01 |
1.23E-16 |
4.08E+14 |
0.00E+00 |
4.08E+14 |
<4% |
100 |
Optical Density >= 50, 5 for sizes >=500nm
|
Wt conc = 2.5mg/mL, 1mL volume typical
|
Wt % = 0.25%
|
| ppm = 2500 |
%PDI = Std Dev/Size
|
See Tech Note TN801 for definition of terms and method of analysis
|
All specs typical. May vary batch to batch. Exact values are measured for each batch
|
Shape monodispersity (% spheres) > 99.9%
|
Solution default is 18MEG DI water
|
Residual Chemicals < 0.1%
|
Part # |
Diameter (nm) |
Length (nm) |
Peak SPR Wave (nm) |
Aspect Ratio |
OD SPR (AU) |
Peak LSPR Wave (nm) |
OD LSPR (AU) |
Nanorods /mL |
Wt. conc (ug/ml) |
Wt. % |
PPM |
Molarity (nM) |
Picomoles |
SPR Molar Ext. (M-1cm-1) |
LSPR Molar Ext. (M-1cm-1) |
Peak SPR (nm) |
SPR Linewidth 80% (nm) |
5-808 |
5 |
21 |
808 |
4.2 |
50 |
510 |
10 |
2.39E+14 |
1750 |
0.18% |
1750 |
398.72 |
3.99E+02 |
1.25E+08 |
2.51E+07 |
794-829 |
100 |
5-780 |
5 |
19 |
780 |
3.8 |
50 |
510 |
10 |
2.67E+14 |
1750 |
0.18% |
1750 |
444.72 |
4.45E+02 |
1.12E+08 |
2.25E+07 |
765-794 |
100 |
5-700 |
5 |
15 |
700 |
3 |
50 |
510 |
10 |
3.47E+14 |
1750 |
0.18% |
1750 |
578.14 |
5.78E+02 |
8.65E+07 |
1.73E+07 |
675-725 |
100 |
10-2100 |
10 |
175 |
2100 |
17.5 |
50 |
510 |
10 |
6.73E+12 |
1750 |
0.18% |
1750 |
11.21 |
1.12E+01 |
4.46E+09 |
8.92E+08 |
1900-2300 |
300 |
10-1400 |
10 |
102 |
1400 |
10.2 |
50 |
510 |
10 |
1.17E+13 |
1750 |
0.18% |
1750 |
19.47 |
1.95E+01 |
2.57E+09 |
5.14E+08 |
1300-1500 |
150 |
10-1200 |
10 |
81 |
1200 |
8.1 |
50 |
510 |
10 |
1.48E+13 |
1750 |
0.18% |
1750 |
24.67 |
2.47E+01 |
2.03E+09 |
4.05E+08 |
1132-1300 |
150 |
10-1064 |
10 |
67 |
1064 |
6.7 |
50 |
510 |
10 |
1.81E+13 |
1750 |
0.18% |
1750 |
30.13 |
3.01E+01 |
1.66E+09 |
3.32E+08 |
1022-1132 |
150 |
10-980 |
10 |
59 |
980 |
5.9 |
50 |
510 |
10 |
2.09E+13 |
1750 |
0.18% |
1750 |
34.91 |
3.49E+01 |
1.43E+09 |
2.86E+08 |
965-1022 |
150 |
10-950 |
10 |
55 |
950 |
5.5 |
50 |
510 |
10 |
2.22E+13 |
1750 |
0.18% |
1750 |
37 |
3.70E+01 |
1.35E+09 |
2.70E+08 |
925-965 |
150 |
10-900 |
10 |
50 |
900 |
5 |
50 |
510 |
10 |
2.47E+13 |
1750 |
0.18% |
1750 |
41.11 |
4.11E+01 |
1.22E+09 |
2.43E+08 |
875-925 |
150 |
10-850 |
10 |
45 |
850 |
4.5 |
50 |
510 |
10 |
2.78E+13 |
1750 |
0.18% |
1750 |
46.25 |
4.63E+01 |
1.08E+09 |
2.16E+08 |
829-875 |
100 |
10-808 |
10 |
41 |
808 |
4.1 |
50 |
510 |
10 |
3.10E+13 |
1750 |
0.18% |
1750 |
51.68 |
5.17E+01 |
9.68E+08 |
1.94E+08 |
794-829 |
75 |
10-780 |
10 |
38 |
780 |
3.8 |
50 |
510 |
10 |
3.36E+13 |
1750 |
0.18% |
1750 |
56.06 |
5.61E+01 |
8.92E+08 |
1.78E+08 |
765-794 |
65 |
10-750 |
10 |
35 |
750 |
3.5 |
50 |
510 |
10 |
3.70E+13 |
1750 |
0.18% |
1750 |
61.67 |
6.17E+01 |
8.11E+08 |
1.62E+08 |
725-765 |
50 |
10-700 |
10 |
29 |
700 |
2.9 |
50 |
510 |
10 |
4.44E+13 |
1750 |
0.18% |
1750 |
74 |
7.40E+01 |
6.76E+08 |
1.35E+08 |
675-725 |
40 |
10-650 |
10 |
24 |
650 |
2.4 |
50 |
510 |
10 |
5.55E+13 |
1750 |
0.18% |
1750 |
92.5 |
9.25E+01 |
5.41E+08 |
1.08E+08 |
625-675 |
40 |
10-600 |
10 |
19 |
600 |
1.9 |
50 |
510 |
10 |
7.40E+13 |
1750 |
0.18% |
1750 |
123.34 |
1.23E+02 |
4.05E+08 |
8.11E+07 |
575-625 |
40 |
25-1400 |
25 |
245 |
1400 |
9.8 |
50 |
514 |
15 |
1.12E+12 |
2500 |
0.25% |
2500 |
1.86 |
1.86E+00 |
2.69E+10 |
8.06E+09 |
1232-1500 |
150 |
25-1064 |
25 |
137 |
1064 |
5.5 |
50 |
514 |
15 |
2.05E+12 |
2500 |
0.25% |
2500 |
3.42 |
3.42E+00 |
1.46E+10 |
4.38E+09 |
1022-1232 |
150 |
25-980 |
25 |
119 |
980 |
4.8 |
50 |
514 |
15 |
2.39E+12 |
2500 |
0.25% |
2500 |
3.98 |
3.98E+00 |
1.26E+10 |
3.77E+09 |
965-1022 |
150 |
25-950 |
25 |
102 |
950 |
4.1 |
50 |
514 |
15 |
2.82E+12 |
2500 |
0.25% |
2500 |
4.7 |
4.70E+00 |
1.06E+10 |
3.19E+09 |
925-965 |
150 |
25-900 |
25 |
96 |
900 |
3.8 |
50 |
514 |
15 |
3.01E+12 |
2500 |
0.25% |
2500 |
5.02 |
5.02E+00 |
9.95E+09 |
2.99E+09 |
875-925 |
150 |
25-850 |
25 |
93 |
850 |
3.7 |
50 |
514 |
15 |
3.12E+12 |
2500 |
0.25% |
2500 |
5.2 |
5.20E+00 |
9.61E+09 |
2.88E+09 |
829-875 |
150 |
25-808 |
25 |
90 |
808 |
3.6 |
50 |
514 |
15 |
3.24E+12 |
2500 |
0.25% |
2500 |
5.39 |
5.39E+00 |
9.27E+09 |
2.78E+09 |
794-829 |
150 |
25-780 |
25 |
87 |
780 |
3.5 |
50 |
514 |
15 |
3.36E+12 |
2500 |
0.25% |
2500 |
5.6 |
5.60E+00 |
8.93E+09 |
2.68E+09 |
765-794 |
150 |
25-750 |
25 |
85 |
750 |
3.4 |
50 |
514 |
15 |
3.45E+12 |
2500 |
0.25% |
2500 |
5.75 |
5.75E+00 |
8.70E+09 |
2.61E+09 |
725-765 |
65 |
25-700 |
25 |
75 |
700 |
3 |
50 |
514 |
15 |
3.96E+12 |
2500 |
0.25% |
2500 |
6.61 |
6.61E+00 |
7.57E+09 |
2.27E+09 |
675-725 |
60 |
25-650 |
25 |
71 |
650 |
2.8 |
50 |
514 |
15 |
4.22E+12 |
2500 |
0.25% |
2500 |
7.03 |
7.03E+00 |
7.11E+09 |
2.13E+09 |
625-675 |
60 |
25-600 |
25 |
57 |
600 |
2.3 |
50 |
514 |
15 |
5.43E+12 |
2500 |
0.25% |
2500 |
9.05 |
9.05E+00 |
5.52E+09 |
1.66E+09 |
575-625 |
60 |
25-550 |
25 |
34 |
550 |
1.4 |
50 |
514 |
15 |
1.01E+13 |
2500 |
0.25% |
2500 |
16.91 |
1.69E+01 |
2.96E+09 |
8.87E+08 |
525-575 |
60 |
40-1064 |
40 |
208 |
850 |
5.2 |
50 |
520 |
20 |
5.30E+11 |
2500 |
0.25% |
2500 |
0.88 |
8.84E-01 |
5.66E+10 |
2.26E+10 |
1022-1232 |
150 |
40-980 |
40 |
180 |
850 |
4.5 |
50 |
520 |
20 |
6.19E+11 |
2500 |
0.25% |
2500 |
1.03 |
1.03E+00 |
4.84E+10 |
1.94E+10 |
875-1022 |
150 |
40-850 |
40 |
148 |
850 |
3.7 |
50 |
520 |
20 |
7.67E+11 |
2500 |
0.25% |
2500 |
1.28 |
1.28E+00 |
3.91E+10 |
1.57E+10 |
829-875 |
150 |
40-808 |
40 |
134 |
808 |
3.4 |
50 |
520 |
20 |
8.56E+11 |
2500 |
0.25% |
2500 |
1.43 |
1.43E+00 |
3.51E+10 |
1.40E+10 |
794-829 |
150 |
40-780 |
40 |
124 |
780 |
3.1 |
50 |
520 |
20 |
9.33E+11 |
2500 |
0.25% |
2500 |
1.55 |
1.55E+00 |
3.22E+10 |
1.29E+10 |
765-794 |
150 |
40-750 |
40 |
112 |
750 |
2.8 |
50 |
520 |
20 |
1.05E+12 |
2500 |
0.25% |
2500 |
1.74 |
1.74E+00 |
2.87E+10 |
1.15E+10 |
725-765 |
150 |
40-700 |
40 |
92 |
700 |
2.3 |
50 |
520 |
20 |
1.31E+12 |
2500 |
0.25% |
2500 |
2.19 |
2.19E+00 |
2.29E+10 |
9.14E+09 |
675-725 |
60 |
40-650 |
40 |
80 |
650 |
2 |
50 |
520 |
20 |
1.55E+12 |
2500 |
0.25% |
2500 |
2.58 |
2.58E+00 |
1.94E+10 |
7.75E+09 |
625-675 |
60 |
40-600 |
40 |
68 |
600 |
1.7 |
50 |
520 |
20 |
1.89E+12 |
2500 |
0.25% |
2500 |
3.15 |
3.15E+00 |
1.59E+10 |
6.35E+09 |
575-625 |
60 |
40-550 |
40 |
60 |
550 |
1.5 |
50 |
520 |
20 |
2.21E+12 |
2500 |
0.25% |
2500 |
3.69 |
3.69E+00 |
1.36E+10 |
5.42E+09 |
525-575 |
60 |
50-1064 |
50 |
245 |
1064 |
4.9 |
50 |
530 |
25 |
2.89E+11 |
2500 |
0.25% |
2500 |
0.48 |
4.82E-01 |
1.04E+11 |
5.18E+10 |
980-1232 |
150 |
50-808 |
50 |
145 |
808 |
2.9 |
50 |
530 |
25 |
5.15E+11 |
2500 |
0.25% |
2500 |
0.86 |
8.58E-01 |
5.83E+10 |
2.91E+10 |
750-850 |
150 |
50-700 |
50 |
110 |
700 |
2.2 |
50 |
530 |
25 |
7.08E+11 |
2500 |
0.25% |
2500 |
1.18 |
1.18E+00 |
4.24E+10 |
2.12E+10 |
650-750 |
150 |
50-600 |
50 |
100 |
600 |
2 |
50 |
530 |
25 |
7.93E+11 |
2500 |
0.25% |
2500 |
1.32 |
1.32E+00 |
3.78E+10 |
1.89E+10 |
550-650 |
150 |
70-750 |
70 |
120 |
750 |
1.7 |
50 |
530 |
25 |
3.49E+11 |
2500 |
0.25% |
2500 |
0.58 |
5.81E-01 |
8.60E+10 |
4.30E+10 |
700-800 |
150 |
70-650 |
70 |
105 |
650 |
1.5 |
50 |
530 |
25 |
4.13E+11 |
2500 |
0.25% |
2500 |
0.69 |
6.88E-01 |
7.27E+10 |
3.63E+10 |
600-700 |
150 |
70-550 |
70 |
91 |
550 |
1.3 |
50 |
530 |
25 |
4.98E+11 |
2500 |
0.25% |
2500 |
0.83 |
8.30E-01 |
6.02E+10 |
3.01E+10 |
500-600 |
150 |
Optical Density >= 50, volume = 1mL
|
All specs typical. May vary batch to batch. Exact values are measured for each batch
|
Solution default is 18MEG DI water
|
Residual Chemicals < 0.1%
|
Nanopartz Zero Order Gold Nanorods
Composition
These nanoparticles are shipped in 18MEG DI water (default solution) with no measurable residual reactants.
Custom Formulation
Please contact us.
Quantity
This product is available in 1mL volumes and larger.
Introductory Kits
Please contact us.
Delivery
Standard sizes are in stock. Special order sizes are shipped in two weeks or less. All domestic shipments are sent Fed Ex Standard Overnight delivery, international Fed Ex Priority 2 day. No shipments on Fridays except for dried particles. Saturday shipping available for extra charge.
Functionalization
This product comes with a number of different options are bare with the silica exposed. The product is stable with silica coating only.
Shelf Life/Storage Temperature
This product is guaranteed for six months and should be stored at 4 °C after opening. Care must be taken to only use sterile glassware when working with this product.
Toxicity
This product is known to be noncytotoxic. This product has been sterilized and is biological free.
Sterilization
This product is sterilized.
For post sterilization testing, please choose Sterilization Certification.
For endotoxin purification, choose Endotoxin Purified.
Certifications
Every order comes with a Certification of Analysis that includes the following information. We use calibration traceable:
UV-VIS (Agilent 8453) for extinction and concentration measurements
NIR (Cary 500) for NIR extinction and concentration measurements
DLS (Malvern Nano ZS) for zeta potential measurement
ICP-MS (Varian 820-MS) for gold mass measurements
TEM (Phillips CM-100 100KV) for sizing
How do I determine the concentration of the product?
Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solution you add.
Why do you sell your rods as OD-mLs and what does this mean?
This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.
Can I resuspend in PBS?
Yes
What is the shelf life if I never open the package and keep refrigerated?
Many years.
Should I store in the refrigerator?
Yes.
How do you size your gold nanoparticles? Does the size include the capping agent?
We use three methods to specify our gold nanoparticles; TEM, UV VIS, and DLS. Each has its own advantages and disadvantages, and we use a weighted system to take advantage of each methods strengths. In the end, we place the strongest weight to the TEM method, particularly since we use samples sizes greater than 50 particles for each lot.
Do you really provide a TEM for my specific lot?
Yes, and not just for 5-10 particles, rather 50-100 are standard.
What is OD?
Optical Density (OD) is measured by UV-VIS. An Optical Density OD=1 corresponds to a transmission of 10% through a 1cm cuvette. Optical Density is a nice unit to use since Optical Densities correlate linearly to concentration. So an Optical Density of 1.2 is equal to 1.2 times the concentration of a gold nanoparticle solution that has an Optical Density of 1. We use OD and concentration interchangingly as it is easier to refer to a solution of OD=1 rather than 2.35e12 nanoparticles. For all spheres up to 200nm, OD=1 does refer to 0.05mg/mL.
What is PDI?
PDI refers to polydispersity index and is equal to the standard deviation of the particle sizes divided by the average size.
“It’s indeed a useful product that could find its way to a number of applications and customers including plasmonic bio-sensing experiments ”
Radwanul Hasan
Siddique Research Group Lead at Samsung R&D
Pasadena, California
- Lin, D. Z., Chuang, P. C., Liao, P. C., and Chen, J. P. "Increasing the Spectral Shifts in LSPR Biosensing Using DNA-Functionalized Gold Nanorods in a Competitive Assay Format for the Detection of Interferon-γ." Biosensors and Bioelectronics, Elsevier, 2016, pp. [include page numbers if available].
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Li, R., Dong, N., Cheng, C., Ren, F., Hübner, R., Wang, J., et al. "Giant Enhancement of Nonlinear Optical Response in Nd:YAG Single Crystals by Embedded Silver Nanoparticles." ACS Omega, vol. 2, no. 5, 2017, American Chemical Society, pp. [include page numbers if available].
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Slaughter, L. S., Chang, W. S., Swanglap, P., et al. "Single-Particle Spectroscopy of Gold Nanorods Beyond the Quasi-Static Limit: Varying the Width at Constant Aspect Ratio." The Journal of Physical Chemistry C, vol. 114, no. 12, 2010, American Chemical Society, pp. [include page numbers if available].
The manufacturing method for these nanoparticles and the ability to measure the silica thicknesses are based on our proprietary method.
| Zero Order Gold Nanoparticles | |
| Stability (salt,pH,chemicals) | High |
| Monovalent | No |
| Nonspecific binding | Very low |
| Sterilization | Yes |
- Covalent bonds insure specificity, stability, long shelf life
- Buffer Stability - stable from pH 4-9
- No sodium azide
- No BSA
- Polymer coating insures no aggregation in high salts, reduced nonspecific binding
- Stable
- Well Characterized
- Customer can select buffer
- Customer can select gold nanoparticle type, size and/or SPR
- Loading of all ligands is optimized
Example part number is PZ11-10-CON-DIH-50-1-CS-EP where:
PZ11 - Product family number for Zero OrderSpherical Gold Nanoparticles. For Gold Nanorods, its PZ12.
10 - Sphere diameter in nanometers. Other choices are 1.8 to 1500
CON - Conjugation, including Protein A, Biotin, and many more. Contact us for custom attachments.
DIH - Buffer, in this case 18MEG DI water. Other choices are PBS, MES, Sodium Borate, TRIS
50 - Optical Density, in this case OD=50, optional 250
1 - Volume (mL). Other choices are 5mL, 10mL and more.
CS - Certified Sterilized - Though the product come sterilized, this option includes testing and certification.
EP - Endotoxin Purified - The product is endotoxin purified and certified.
Ordering by scrolling down and selecting the options from the selection below.